Abstract

Nucleotide sequences of the circadian rhythm genes, period and timeless, were studied for the first time in mosquitoes Culex pipiens Linnaeus, 1758. In this work we evaluated variations of the studied genome fragments for the two forms of C. pipiens (forma “pipiens” – mosquitoes common for aboveground habitats, forma “molestus” – underground mosquitoes). We compared C. pipiens from Russia with transatlantic C. pipiens and subtropical Culex quinquefasciatus Say, 1823. Our results show that intraspecies variability is higher for the gene period than for the gene timeless. The revealed substitutions in nucleotide sequences and especially in amino acid sequences grouped the individuals of the two forms into distinct clusters with high significance. The detected fixed amino acid substitutions may appear essential for functioning of the circadian rhythm proteins in C. pipiens, and may be correlated with adaptations of the taxa within the group C. pipiens. Our results suggest that natural selection favors fixed mutations and the decrease in diversity of the genes period and timeless in mosquitoes of the C. pipiens f. “molestus” compared with the C. pipiens f. “pipiens”, is probably correlated with adaptive features of C. pipiens f. “molestus”. The studied genome regions may be considered as promising molecular-genetic markers for identification, population and phylogenetic analysis of similar species and forms of the Culex pipiens complex.

Addeddate

2025-03-16 14:43:42

Bhl_virtual_titleid

210879

Bhl_virtual_volume

v.10:no.4 (2016)

Call number

10_3897_CompCytogen_v10i4_7582

Call-number

10_3897_CompCytogen_v10i4_7582

Foldoutcount

0

Genre

article

Identifier

comparativeanal10shai

Identifier-ark

ark:/13960/s232wkz3v9h

Identifier-bib

10_3897_CompCytogen_v10i4_7582

Identifier-doi

10.3897/CompCytogen.v10i4.7582

Issue

4

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

85

Page_number_module_version

1.0.5

Page_range

483-504

Pages

22

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Comparative Cytogenetics 10(4)

Year

2016

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